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human breast cancer cell lines t47d (ATCC)

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ATCC human breast cancer cell lines t47d
Human Breast Cancer Cell Lines T47d, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+lines+t47d/T-47D/10__31083_slash_fbl45389-51-0-28
Average 99 stars, based on 6848 article reviews

human breast cancer cell lines t47d - by Bioz Stars, 2026-09

99/100 stars

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Multiple Displacement Amplification:

Article Title: Development of the First Small-Molecule Inhibitor Targeting Oncostatin M for Treatment of Breast Cancer
Article Snippet: Protein used: gp130 (R&D Systems, cat. #671-GP) and OSMRβ (R&D Systems, cat. # 4389-OR). .. Human breast cancer cell lines T47D, MDA-MB-231, and MCF-7 were obtained from the American Type Culture Collection (ATCC; Manassas, VA). .. All cell lines were maintained in RPMI-1640 media (Genesee Scientific; San Diego, CA) supplemented with either 10% fetal bovine serum or 10% synthetic Fetal Clone III serum (Thermo Fisher; Waltham, MA) and 1% penicillin/streptomycin (Genesee Scientific).

Article Title: The Role of Nesprin-4 in Breast Cancer Migration and Invasion.
Article Snippet: .. Human breast cancer cell lines T47D, MCF7, and MDA-MB-231 (#HTB-133, #HTB-22, and #CRM-HTB-26, respectively, from ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum, 1% (v/v) L-glutamine, and 1% (v/v) penicillin and streptomycin. ..

Article Title: SB431542, a Selective Inhibitor of the TGF-β Type I Receptor, Enhances Doxorubicin Antitumor Activity via p63 Activation in Mutant p53 Breast Cancer Cells
Article Snippet: .. Human breast cancer cell lines T47D, MDA-MB231 and BT-474 were obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan), while MDA-MB-468 cells were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA). ..

Article Title: The Role of Nesprin-4 in Breast Cancer Migration and Invasion
Article Snippet: .. Human breast cancer cell lines T47D, MCF7, and MDA-MB-231 (#HTB-133, #HTB-22, and #CRM-HTB-26, respectively, from ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% ( v / v ) fetal bovine serum, 1% ( v / v ) L-glutamine, and 1% ( v / v ) penicillin and streptomycin. ..

Article Title: Obesity-Related Inflammation Reduces Treatment Sensitivity and Promotes Aggressiveness in Luminal Breast Cancer Modulating Oxidative Stress and Mitochondria
Article Snippet: 17β-estradiol, leptin, IL-6, TNF-α, Tamoxifen, and Paclitaxel were obtained from Merck (St. Louis, MO, USA). .. Human breast cancer cell lines T47D and MCF7 (luminal) and MDA-MB-231 (triple-negative) were obtained from American Type Culture Collection ATCC (Manassas, VA, USA). .. Biowest (Riverside, MO, USA) supplied Dulbecco’s modified Biomedicines 2024, 12, 2813 3 of 15 Eagle’s medium (DMEM) High Glucose, pH 7.20 ± 0.30, while GIBCO (Paisley, UK) supplied DMEM without phenol red, pH 7.20 ± 0.20.

Article Title: Non-redundant roles of the phosphoinositide phosphatases PTEN and PIPP in PI3K/AKT signaling in breast cancer.
Article Snippet: The data was analyzed using QuantaSoft software (Bio-Rad) with the thresholds for detection set manually based on the results from the no template control. .. Culture of human breast cancer cell lines T47D (# HTB-133), MDA-MB-231 (# HTB-26), Hs578T, MCF-7, ZR-75-1, SKBR3 and BT549 human breast cancer cells and MCF-10A human mammary epithelial cells were purchased from American Type Culture Collections (Manassas, VA). .. SUM149PT and SUM185PE were purchased from Asterand Bioscience.

Cell Culture:

Article Title: MCL-1 as a molecular switch between myofibroblastic and pro-angiogenic features of breast cancer-associated fibroblasts
Article Snippet: .. The human breast cancer cell lines T47D were purchased from American Type Culture Collection (Bethesda, MD, USA) and was cultured in RPMI medium supplemented with 10% FBS and 2 mM glutamine. ..

Modification:

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Synergistic cytotoxic effect of 17-AAG-loaded PEG-CS nanoparticles and trastuzumab on T47D cells. Cell viability was assessed by MTT assay after 72 h of treatment. Data are presented as mean ± SD ( n = 3). The combination group (17-AAG-loaded NPs + trastuzumab) showed significantly reduced cell viability (15%) compared to treatment with 17-AAG-loaded NPs alone (50%), trastuzumab alone (35%), or blank NPs + trastuzumab (100%). Statistical significance: **** p < 0.0001 vs. Blank NPs + Trastuzumab group (one-way ANOVA with Tukey’s post-hoc test). The Combination Index (CI) calculated using CompuSyn software was 0.72, indicating strong synergy.

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Synergistic cytotoxic effect of 17-AAG-loaded PEG-CS nanoparticles and trastuzumab on T47D cells. Cell viability was assessed by MTT assay after 72 h of treatment. Data are presented as mean ± SD ( n = 3). The combination group (17-AAG-loaded NPs + trastuzumab) showed significantly reduced cell viability (15%) compared to treatment with 17-AAG-loaded NPs alone (50%), trastuzumab alone (35%), or blank NPs + trastuzumab (100%). Statistical significance: **** p < 0.0001 vs. Blank NPs + Trastuzumab group (one-way ANOVA with Tukey’s post-hoc test). The Combination Index (CI) calculated using CompuSyn software was 0.72, indicating strong synergy.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: MTT Assay, Software

In vitro cytotoxicity of 17-AAG-loaded PEG-CS NPs in T47D and MDA-MB-231 cells. Cell viability after 72 h treatment with free 17-AAG or 17-AAG-loaded NPs. The nanoformulation showed significantly enhanced cytotoxicity, with an IC₅₀ of 2.3 ± 0.3 µM in T47D cells, representing a threefold increase compared to free 17-AAG (IC₅₀ = 6.9 ± 0.5 µM). Similar results were observed in MDA-MB-231 cells (IC₅₀ = 3.0 µM for NPs vs. 8.1 µM for free drug). Blank PEG-CS NPs showed no significant toxicity in NHDF cells (up to 50 µg/mL). Data are mean ± SEM ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test. **** P < 0.0001 vs. control.

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: In vitro cytotoxicity of 17-AAG-loaded PEG-CS NPs in T47D and MDA-MB-231 cells. Cell viability after 72 h treatment with free 17-AAG or 17-AAG-loaded NPs. The nanoformulation showed significantly enhanced cytotoxicity, with an IC₅₀ of 2.3 ± 0.3 µM in T47D cells, representing a threefold increase compared to free 17-AAG (IC₅₀ = 6.9 ± 0.5 µM). Similar results were observed in MDA-MB-231 cells (IC₅₀ = 3.0 µM for NPs vs. 8.1 µM for free drug). Blank PEG-CS NPs showed no significant toxicity in NHDF cells (up to 50 µg/mL). Data are mean ± SEM ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test. **** P < 0.0001 vs. control.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: In Vitro, Control

Flow cytometric analysis of apoptosis in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. A Untreated control cells. B Cells treated with 17-AAG-loaded PEG-CS NPs. The percentage of apoptotic cells (Q2 + Q3) increased from 16.5% in the control group to 52.2% in the treated group. Data are representative of three independent experiments ( n = 3).

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Flow cytometric analysis of apoptosis in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. A Untreated control cells. B Cells treated with 17-AAG-loaded PEG-CS NPs. The percentage of apoptotic cells (Q2 + Q3) increased from 16.5% in the control group to 52.2% in the treated group. Data are representative of three independent experiments ( n = 3).

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Control

Cell cycle distribution in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. The treated group showed an increase in the G2/M phase (26.50%) compared to control (19.30%), indicating G2/M arrest. Data are representative of three independent experiments ( n = 3).

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Cell cycle distribution in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. The treated group showed an increase in the G2/M phase (26.50%) compared to control (19.30%), indicating G2/M arrest. Data are representative of three independent experiments ( n = 3).

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Control

qRT-PCR analysis of HSP90 and β-actin gene expression in T47D cells after 72 h treatment with 17-AAG-loaded PEG-CS NPs. A Melting curves for HSP90 (Tm = 78.2 °C) and β-actin (Tm = 83.9 °C), showing single sharp peaks that confirm the specificity of amplification and absence of primer-dimers. Data are representative of three independent experiments ( n = 3). B Amplification curves for HSP90 (purple line) and β-actin (blue line) genes, showing the cycle threshold (Ct) values used for relative quantification of gene expression.

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: qRT-PCR analysis of HSP90 and β-actin gene expression in T47D cells after 72 h treatment with 17-AAG-loaded PEG-CS NPs. A Melting curves for HSP90 (Tm = 78.2 °C) and β-actin (Tm = 83.9 °C), showing single sharp peaks that confirm the specificity of amplification and absence of primer-dimers. Data are representative of three independent experiments ( n = 3). B Amplification curves for HSP90 (purple line) and β-actin (blue line) genes, showing the cycle threshold (Ct) values used for relative quantification of gene expression.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Quantitative RT-PCR, Gene Expression, Amplification, Quantitative Proteomics

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human breast cancer cell lines t47d (ATCC)

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